![]() ![]() ![]() This would enable "fast photon counting" with the four SMD HyD's (8x faster counting than now). Ideally purchase FOUR SMD HyD's and negotiate trade-in of the current two 2nd gen HyD's. If you have money, and especially if you need speed and/or plex, please consider purchasing SMD HyD's for this SP8 confocal microscope. Our HyD's are "2nd generation", in mid-2018 Leica introduced single molecule detection SMD HyD's, that each count 2x faster than our 2nd gen HyD's. Our SP8 has space for two more HyD's internally.This enables much simpler quantitation of fluorescence signals than conventional PMTs whose digitizers output values that depend on both the High Voltage ("HV") gain and the "offset". The two Leica HyD detectors are best operated in "counting mode" = photon counting. with 3 detectors, can acquire many channels by sequential scanning multiple tracks). Our Lecia SP8 confocal microscope was purchased on a budget, so between the price and capabilities of a 'baby confocal' like Leica SPE, and a 'fully equipped' confocal microscope (example: our SP8 has only 3 detectors, no incubator. for assistance with the ACCM confocal microscope (routine training and question answering does not rate acknowledgement). If you get 'significant' assistance mage core staff: We thank George McNamara, Ph.D. Microscope: We acknowledge the Department of Anesthesiology & Critical Care Medicine (ACCM) for access to their Leica SP8 confocal microscope. Bin Wu, image core manager George McNamara, PhD, 30 (cell), text for Acknowledgements: This microscope is owned by the Department of Anesthesiology & Critical Care Medicine (ACCM) and managed by Ross Fluorescence Imaging Center, - Director Prof. If a user needs their PI or administrator to set up account numbers (IO#'s in JHU accounting jargon), see Jwas the start of the iLabs Organizer reservations for our Leica SP8 confocal microscope: ACCM Leica SP8 Confocal Microscope (ACCM Confocal Microscope) we want ACCM staff to be able to use at least 20 of the ~40 hour work week hours). * open access to JHU (and outside) researchers - if heavily booked, ACCM staff will have priority access (i.e. ![]() The Leica SP8 confocal microscope is on a Leica DMi8 inverted microscope stand (DMi8CEL). ![]()
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